| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 北市大學 | 1195洪雋崴 | 03:58.01 | 50M:27.50, 100M:57.67, 150M:1:28.05, 200M:1:58.99, 250M:2:29.42, 300M:2:59.82, 350M:3:29.65, 400M:3:58.01 | q01 | |
| 臺灣體大 | 1154王星皓 | 03:58.96 | 50M:27.17, 100M:57.43, 150M:1:27.99, 200M:1:59.28, 250M:2:30.05, 300M:3:00.55, 350M:3:29.82, 400M:3:58.96 | q02 | |
| 北市大學 | 1194洪本翰 | 03:59.95 | 50M:27.61, 100M:57.65, 150M:1:27.99, 200M:1:58.91, 250M:2:29.78, 300M:2:59.94, 350M:3:30.02, 400M:3:59.95 | q03 | |
| 國立體大 | 1160廖梓丞 | 04:10.27 | 50M:28.14, 100M:58.94, 150M:1:30.06, 200M:2:01.77, 250M:2:33.28, 300M:3:05.55, 350M:3:38.13, 400M:4:10.27 | q05 | |
| 東華大學 | 1033黃國倫 | 04:12.79 | 50M:28.34, 100M:59.63, 150M:1:31.50, 200M:2:04.32, 250M:2:36.78, 300M:3:09.35, 350M:3:41.36, 400M:4:12.79 | q06 | |
| 北市大學 | 1184張虹恩 | 04:18.09 | 50M:29.39, 100M:1:00.90, 150M:1:33.66, 200M:2:06.20, 250M:2:39.12, 300M:3:12.12, 350M:3:46.06, 400M:4:18.09 | q07 | |
| 高雄大學 | 1068戴立喆 | 04:18.11 | 50M:28.80, 100M:1:00.65, 150M:1:32.27, 200M:2:05.12, 250M:2:38.82, 300M:3:13.34, 350M:3:46.70, 400M:4:18.11 | q08 | |
| 虎尾科大 | 1041陳禹亨 | 04:25.82 | 50M:28.69, 100M:59.39, 150M:1:31.56, 200M:2:04.66, 250M:2:38.45, 300M:3:13.65, 350M:3:49.65, 400M:4:25.82 | | |