| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 臺灣體大 | 賴秉杰,陳義翔,馬家彥,傅堃銘 | 04:01.60 | 50M:29.86, 100M:1:02.21, 150M:1:32.72, 200M:2:09.02, 250M:2:35.80, 300M:3:07.76, 350M:3:33.27, 400M:4:01.60 | q01 | |
| 海洋大學 | 李天曦,白奐恩,張浩瀚,蘇柏綸 | 04:07.60 | 50M:30.40, 100M:1:03.49, 150M:1:35.90, 200M:2:13.68, 250M:2:40.21, 300M:3:12.19, 350M:3:38.50, 400M:4:07.60 | q02 | |
| 臺灣師大 | 洪子恩,許立諭,謝季儒,阮柏堯 | 04:07.78 | 50M:29.71, 100M:1:02.00, 150M:1:33.50, 200M:2:09.76, 250M:2:38.10, 300M:3:13.30, 350M:3:39.15, 400M:4:07.78 | q03 | |
| 北市大學 | 藍奕峰,陳仲澤,洪亞新,張軒誠 | 04:08.73 | 50M:28.86, 100M:1:01.44, 150M:1:32.41, 200M:2:09.46, 250M:2:35.53, 300M:3:06.36, 350M:3:38.41, 400M:4:08.73 | q04 | |
| 國北教大 | 劉彥呈,江承恩,呂宜憲,洪士膺 | 04:09.15 | 50M:30.17, 100M:1:03.56, 150M:1:36.00, 200M:2:12.93, 250M:2:40.32, 300M:3:14.36, 350M:3:40.03, 400M:4:09.15 | q05 | |
| 虎尾科大 | 陳麒鈞,陳科均,吳科緯,陳禹亨 | 04:10.77 | 50M:30.58, 100M:1:04.74, 150M:1:36.98, 200M:2:15.03, 250M:2:43.41, 300M:3:15.83, 350M:3:42.09, 400M:4:10.77 | q06 | |
| 陽明交大 | 胡翔發,方淳浲,余定綸,朱皓伸 | 04:11.41 | 50M:30.97, 100M:1:05.08, 150M:1:37.35, 200M:2:15.79, 250M:2:43.23, 300M:3:15.55, 350M:3:41.92, 400M:4:11.41 | q07 | |
| 高雄大學 | 林佑達,苗農群,戴立喆,洪文傑 | 04:11.78 | 50M:31.44, 100M:1:05.32, 150M:1:37.84, 200M:2:16.24, 250M:2:43.18, 300M:3:15.47, 350M:3:42.73, 400M:4:11.78 | q08 | |
| 中正大學 | 黃梓境,董俊佑,張睿晨,李 易 | 04:13.75 | 50M:32.07, 100M:1:07.03, 150M:1:39.67, 200M:2:19.09, 250M:2:47.06, 300M:3:19.01, 350M:3:44.95, 400M:4:13.75 | | |
| 臺灣科大 | 侯柏宇,劉景閎,柯建丞,蕭仲廷 | 04:16.48 | 50M:30.78, 100M:1:04.02, 150M:1:36.74, 200M:2:12.81, 250M:2:40.89, 300M:3:15.70, 350M:3:44.17, 400M:4:16.48 | | |
| 清華大學 | 邵楷閎,李恩愷,蕭永淇,陳明宏 | 04:17.62 | 50M:30.20, 100M:1:02.65, 150M:1:38.06, 200M:2:19.80, 250M:2:47.78, 300M:3:21.40, 350M:3:47.44, 400M:4:17.62 | | |
| 輔仁大學 | 鄒家琪,陳立宇,陳冠豪,蘇上紘 | 04:18.59 | 50M:31.30, 100M:1:07.31, 150M:1:40.19, 200M:2:19.21, 250M:2:47.79, 300M:3:23.09, 350M:3:49.33, 400M:4:18.59 | | |
| 逢甲大學 | 王雋升,魏千凱,洪郁修,廖晉德 | 04:22.24 | 50M:32.17, 100M:1:07.19, 150M:1:39.80, 200M:2:16.82, 250M:2:45.84, 300M:3:19.85, 350M:3:48.19, 400M:4:22.24 | | |
| 高雄師大 | 許勝佑,陳柏宇,牟善竹,田鎮睿 | 04:24.36 | 50M:33.19, 100M:1:09.27, 150M:1:42.52, 200M:2:20.65, 250M:2:49.33, 300M:3:24.22, 350M:3:50.89, 400M:4:24.36 | | |
| 屏東大學 | 張玟諺,曾翊瑞,田鎮維,林秉頡 | | | | 請假 |